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Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.
Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Our Abpromise guarantee covers the use of ab33252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.|
|Flow Cyt||1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab33252 has not yet been referenced specifically in any publications.