Anti-HLA Class I 抗体 [YTH862.2] (ab33252)


  • 製品名Anti-HLA Class I antibody [YTH862.2]
    HLA Class I 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [YTH862.2] to HLA Class I
  • 特異性ab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.

  • エピトープab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules.
  • ポジティブ・コントロール
    • Human peripheral blood lymphocytes
  • 特記事項

    Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab33252 was purified from tissue culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名YTH862.2
  • アイソタイプIgG2b
  • 研究分野


Our Abpromise guarantee covers the use of ab33252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


Anti-HLA Class I antibody [YTH862.2] 画像

  • FACS analysis of HLA class I expressing cells in human peripheral blood lymphocytes using ab33252.

Anti-HLA Class I antibody [YTH862.2] (ab33252) 使用論文

ab33252 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Infarcted Mouse Heart injected /w or w/o human mon)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Infarcted Mouse Heart injected /w or w/o human mon
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde

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投稿 Jul 20 2017

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I am very pleased to hear you would like to accept our offer and test ab33252 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer...

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Thank you for your phone call. As we discussed, the best staining on mouse tissue will be obtained using a primary antibody raised in a species other than mouse in combination with a secondary antibody that has been pre-adsorbed to remove cross-reactiv...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

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投稿 Jun 17 2010