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Tissue/ cell preparation - Membrane of human tonsil cells.
The antibody recognises virtually all nucleated human cells, it is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.
Our Abpromise guarantee covers the use of ab23755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
Paraformaldehyde or methanol fixed cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||1/250. Use under non reducing condition. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|Functional Studies||Use at an assay dependent concentration.|