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Tissue/ cell preparation of membrane of tonsil cells (Human).
Our Abpromise guarantee covers the use of ab22432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.
See Cabrera CM et al reference.
ab22432 at a 1/100 dilution staining unpermeabilized human HeLa cells (cervical carcinoma) by Immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 10% serum and incubated with the antibody for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.
This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.