Anti-HLA Class I A3 抗体 [7g7h8] (ab86597)

製品の概要

  • 製品名
    Anti-HLA Class I A3 antibody [7g7h8]
    HLA Class I A3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7g7h8] to HLA Class I A3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HLA Class I A3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; U937. Abcam recommends using 3% milk as a blocking agent.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    7g7h8
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Use 3% milk as a blocking agent.

ターゲット情報

  • 関連性
    Involved in the presentation of foreign antigens to the immune system.
  • 細胞内局在
    Type I membrane protein
  • 参照データベース
  • 別名
    • Histocompatibility antigen antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A3 alpha chain antibody
    • HLAA antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A3 antibody
    see all

画像

  • All lanes : Anti-HLA Class I A3 antibody [7g7h8] (ab86597) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 20 minutes

プロトコール

参考文献

ab86597 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.

Lab has confirmed that they have not determined the endotoxin level of antibody ab33640 and ab86597. This means we will not bwe able to confirm how the antibodies will perform in cell culture experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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