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HLA class 2 antigen DQ6 (Human)
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Our Abpromise guarantee covers the use of ab42130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.|
|Microcytotoxicity testing||Use at an assay dependent concentration.
Add 0.9ml of 1% BSA in PBS to 100µl of 10X antibody stock solution.
|Functional Studies||Use at an assay dependent concentration.
Cell Typing, Tissue Staining and Chimerism Studies.
ab42130 has not yet been referenced specifically in any publications.