The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
Also suitable for Cell Typing, Tissue Staining and Chimerism Studies.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HLA A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane.
Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa.
Cell Membrane : single-pass type I membrane protein.