製品の概要
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製品名Anti-HLA B7 antibody [BB7.1]
HLA B7 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [BB7.1] to HLA B7
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由来種Mouse
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アプリケーション適用あり: Flow Cytmore details
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種交差性交差種: Human, Monkey
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免疫原
Full length protein (Human). Papain solubilised HLA-A2, B7
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特記事項
Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: PBS -
Concentration information loading... -
精製度Protein G purified
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一次抗体 備考Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.
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ポリ/モノモノクローナル
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クローン名BB7.1
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ミエローマNS1
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アイソタイプIgG1
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軽鎖の種類unknown
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab20169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | 1/10. Neat - 1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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関連性HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described.
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細胞内局在Cell Membrane, single pass type I membrane protein.
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参照データベース
- Entrez Gene: 3106 Human
- Omim: 142830 Human
- SwissProt: P01889 Human
- Unigene: 77961 Human
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別名
- Abacavir hypersensitivity, susceptibility to antibody
- Drug-induced liver injury due to flucloxacillin antibody
- HLA B antibody
see all
画像
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![Flow Cytometry - Anti-HLA B7 antibody [BB7.1] (ab20169)](http://a.abcam.com/ps/products/20/ab20169/Images/HLA-B7-Primary-antibodies-ab20169-2.jpg)
Flow Cytometry - Anti-HLA B7 antibody [BB7.1] (ab20169)Overlay histogram showing Jurkat cells stained with ab20169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (a20169, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
参考文献
This product has been referenced in:
- Brodsky FM et al. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3-61 (1979). Read more (PubMed: 95015) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"