Anti-HLA B27 抗体 [HLA-ABC-m3] (FITC) (ab23840)

製品の概要

  • 製品名Anti-HLA B27 antibody [HLA-ABC-m3] (FITC)
    HLA B27 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HLA-ABC-m3] to HLA B27 (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab23840 is a cytotoxic antibody which detects HLA B27 alloantigen.
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Immune complex precipitated from HLA B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

  • 特記事項


    ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)ab23840 is a purified IgG prepared by ion exchange chromatography.
  • 一次抗体 備考ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
  • ポリ/モノモノクローナル
  • クローン名HLA-ABC-m3
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18776939

ターゲット情報

  • 関連性Involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
  • 細胞内局在Single-pass type 1 membrane protein
  • 参照データベース
  • 別名
    • HLAB antibody
    • Leukocyte antigen class I B antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I B antibody
    • SPDA1 antibody
    see all

Anti-HLA B27 antibody [HLA-ABC-m3] (FITC) (ab23840) 使用論文

This product has been referenced in:
  • de Jong MA  et al. TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest 118:3440-3452 (2008). ICC/IF ; Human . Read more (PubMed: 18776939) »
  • Orr K  et al. Utilization of commercial antisera and flow cytometry in HLA-B27 typing. Cytometry 18:17-20 (1994). Read more (PubMed: 8082482) »

See all 3 Publications for this product

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The number of smaples can we worked out from the information on the datasheet, 250ul of antibody is provided. The amountof tess in can do will very much depend on the dilution of antibody that you use, which may r...

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Thank you very much for sending the images. It really helps us understand the problem. I can see from the protocol sent that peripheral blood mononuclear cells were used for the ICC assay. It is a very mixed heterogeneous population, containing differe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"