Anti-HLA A2 抗体 [BB7.2] (ab74674)

製品の概要

  • 製品名Anti-HLA A2 antibody [BB7.2]
    HLA A2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BB7.2] to HLA A2
  • アプリケーション適用あり: IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    HLA-A2 solubilised by papain

  • エピトープThe C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein-A affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名BB7.2
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
  • 細胞内局在Membrane; Single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • Antigen presenting molecule antibody
    • HLA A antibody
    • HLA A histocompatibility type antibody
    • HLA antibody
    • HLA class I antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A2 alpha chain antibody
    • HLA-A antibody
    • HLAA antibody
    • Human leukocyte antigen antibody
    • Leucocyte antigen class I antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • Major histocompatibility complex class I antibody
    • MHC class I antigen [precursor] antibody
    • MHC class I antigen A antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A2 antibody
    • MHC class I antigen HLA A antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC leukocyte antigen antibody
    see all

Anti-HLA A2 antibody [BB7.2] 画像

  • Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-HLA A2 antibody [BB7.2] (ab74674) 使用論文

ab74674 has not yet been referenced specifically in any publications.

Product Wall

Code de remise :xxxxxxx
Date d’expiration : 08/04/12

Je suis ravie que vous souhaitiez participer a notre offre et tester ab74674 en western blot. Le code de réduction correspondant est xxxxxxxxx. Ce code de réductio...

Read More

Merci de votre intérêt pour ab74674. A ma connaissance, cet anticorps n’a pas encore été testé en western blot et nous n’offrons pas d’échantillon gratuit à des fins de tests prél...

Read More

Thank you for contacting us.

Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"