Anti-HLA A2 抗体 [BB7.2] - BSA and Azide free (ab74674)
Key features and details
- Mouse monoclonal [BB7.2] to HLA A2 - BSA and Azide free
- Suitable for: IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-HLA A2 antibody [BB7.2] - BSA and Azide free
HLA A2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BB7.2] to HLA A2 - BSA and Azide free -
由来種
Mouse -
特異性
This product has been purified from tissue culture supernatant. -
アプリケーション
適用あり: IP, Flow Cytmore details
適用なし: WB -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human HLA A2. HLA-A2 solubilised by papain Full length Native protein
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エピトープ
The C-terminus of alpha-2 helix and a turn on one of the underlying beta strands. -
特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
BB7.2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74674の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use 2µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use 2µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 3105 Human
- Omim: 142800 Human
- SwissProt: P04439 Human
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別名
- Antigen presenting molecule antibody
- HLA A antibody
- HLA A histocompatibility type antibody
see all
画像
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Flow cytometry of human peripheral blood cells with ab74674 at 2 µg/ml
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Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
データシートおよび資料
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Datasheet download
参考文献 (8)
ab74674 は 8 報の論文で使用されています。
- Chen Z et al. T and B cell Epitope analysis of SARS-CoV-2 S protein based on immunoinformatics and experimental research. J Cell Mol Med 25:1274-1289 (2021). PubMed: 33325143
- Choi J et al. Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations. Cell Rep Methods 1:100084 (2021). PubMed: 35474673
- Pearson CS et al. Identification of a critical sulfation in chondroitin that inhibits axonal regeneration. Elife 7:N/A (2018). PubMed: 29762123
- Li D et al. Development of a T-cell Receptor Mimic Antibody against Wild-Type p53 for Cancer Immunotherapy. Cancer Res 77:2699-2711 (2017). PubMed: 28363997
- Shafaie S et al. Influence of surface geometry on the culture of human cell lines: A comparative study using flat, round-bottom and v-shaped 96 well plates. PLoS One 12:e0186799 (2017). PubMed: 29084285
- Soysal SD et al. Identification and translational validation of novel mammaglobin-A CD8 T cell epitopes. Breast Cancer Res Treat 147:527-37 (2014). PubMed: 25212176
- Tomita Y et al. Peptides derived from human insulin-like growth factor-II mRNA binding protein 3 can induce human leukocyte antigen-A2-restricted cytotoxic T lymphocytes reactive to cancer cells. Cancer Sci 102:71-8 (2011). PubMed: 21087352
- Qu C et al. MHC class I/peptide transfer between dendritic cells overcomes poor cross-presentation by monocyte-derived APCs that engulf dying cells. J Immunol 182:3650-9 (2009). PubMed: 19265143