製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration. PubMed: 25653431
ELISA Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 1 kDa.

ターゲット情報

  • 関連性HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • 細胞内局在Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • 別名
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Anti-HIV1 tat antibody [N3] 画像

  • Anti-HIV1 tat antibody [N3] (ab63957) + Recombinant HIV1 Tat
  • IHC-P image of HIV1 tat staining on Transgenic mouse olfactory bulb. Heat mediated antigen retrieval was performed using trisodium citrate (pH 6). The tissue section was permeabilization using Triton x100. To block non-specififc binding, sections were blocked in 2% BSA in PBS.

    See Abreview

Anti-HIV1 tat antibody [N3] (ab63957) 使用論文

This product has been referenced in:
  • Ozaki T  et al. Delivery of Topically Applied Calpain Inhibitory Peptide to the Posterior Segment of the Rat Eye. PLoS One 10:e0130986 (2015). ELISA, IHC ; Rat . Read more (PubMed: 26107400) »
  • Yoon CH  et al. p53-derived host restriction of HIV-1 replication by protein kinase R-mediated Tat phosphorylation and inactivation. J Virol 89:4262-80 (2015). ICC . Read more (PubMed: 25653431) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample HIV Recombinant protein (recombinant bacterial protein with TAT-domain and)
Loading amount 4 µg
Specification recombinant bacterial protein with TAT-domain and
Treatment IPTG
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Stefan Krautwald

Verified customer

投稿 Mar 09 2012

Thank you for contacting us and reporting the problems encountered with using Anti-HIV1 tat antibody [N3] (ab63957).

We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: trisodium citrate at pH 6.0
Permeabilization Yes - Tritonx100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"