製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 20651230
ChIP Use at an assay dependent concentration. PubMed: 22073215
CHIPseq Use at an assay dependent concentration. PubMed: 22073215
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

Use at an assay dependent concentration.

ELISA 1/10000.

ターゲット情報

  • 関連性
    HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • 細胞内局在
    Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • 別名
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Anti-HIV1 tat antibody - ChIP Grade 画像

  • All lanes : Anti-HIV1 tat antibody - ChIP Grade (ab43014) at 1/2000 dilution

    Lane 1 : Jurkat whole cell lysate 0 hours after infection
    Lane 2 : Jurkat whole cell lysate 2 hours after infection
    Lane 3 : Jurkat whole cell lysate 4 hours after infection
    Lane 4 : Jurkat whole cell lysate 6 hours after infection
    Lane 5 : Jurkat whole cell lysate 8 hours after infection
    Lane 6 : Jurkat whole cell lysate 10 hours after infection

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa

    This image is courtesy of an anonymous Abreview

    Jurkat cells were pNL4.3 infected and samples taken at 2 hour intervals for blotting.

    See Abreview

  • Immunohistochemical analysis of normal mouse brain, labeling HIV1 tat with ab43014. Samples were fixed in 4% paraformaldehyde overnight, cryoprotected in 30% sucrose and frozen at -80°C. 10µm thick frozen coronal sections were blocked in 5% fetal bovine serum, 1% Triton X-100, 0.5% Tween 20 and 1% skim milk in PBS for 2 hours at RT. Samples were incubated with ab43014 overnight at 4°C, and an alexa fluor conjugated secondary antibody for 2 hours at RT. 

Anti-HIV1 tat antibody - ChIP Grade (ab43014) 使用論文

This product has been referenced in:
  • Lee FH  et al. Disrupting GluA2-GAPDH Interaction Affects Axon and Dendrite Development. Sci Rep 6:30458 (2016). IHC-P ; Mouse . Read more (PubMed: 27461448) »
  • Romani B  et al. HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin. Sci Rep 6:31924 (2016). Read more (PubMed: 27550312) »

See all 9 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
10 µg
Treatment
Transfected with HIV-1 NL4-3 for 48 hours
Specification
HEK293T cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 19 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (gel 15%)
Sample
Human Cell lysate - whole cell (astrocyte U251)
Specification
astrocyte U251
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 15 2013

Thank you very much for your email. My colleague is out of the office but I'll be happy to assist you with this issue.

I've looked at our stock, and unforunately we only have the same lot that did not work well for you. Did the replacement vi...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply and for providing the additional information.

It appears that the antibody isn't working as expected, so I would like to offer you a free of charge replacement or credit note. We only have the same lot in stock in our...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Wha...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat Cells)
Loading amount
25 µg
Specification
Jurkat Cells
Treatment
pNL4.3 infected
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 01 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.



I am sorry this product did not perform as stated on the datasheet and for the inconvenie...

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DISCOUNT CODE: **********
Expiration date: **************

I am very pleased to hear you would like to accept our offer and test ab43014 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem thi...

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Thank you for your patience. I have enquired at the originator of the product but unfortunately, this product has not been tested against human macrophages and HIV strain BAL. Therefore, we do not know if it will be suitable for your research. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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