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Recombinant full length protein expressed in E.coli.
Our Abpromise guarantee covers the use of ab43014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20651230|
|ChIP||Use at an assay dependent concentration. PubMed: 22073215|
|CHIPseq||Use at an assay dependent concentration. PubMed: 22073215|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
|ELISA||Use at an assay dependent concentration.|
Immunohistochemical analysis of normal mouse brain, labeling HIV1 tat with ab43014. Samples were fixed in 4% paraformaldehyde overnight, cryoprotected in 30% sucrose and frozen at -80°C. 10µm thick frozen coronal sections were blocked in 5% fetal bovine serum, 1% Triton X-100, 0.5% Tween 20 and 1% skim milk in PBS for 2 hours at RT. Samples were incubated with ab43014 overnight at 4°C, and an alexa fluor conjugated secondary antibody for 2 hours at RT.
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