The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/500. Use Streptavidin (HRP) conjugated detection reagent.
Flow Cyt: 1/200. Use Streptavidin-FITC conjugated detection reagent.
WB: 1/1000. Predicted molecular weight: 14 kDa. Use Streptavidin (HRP) conjugated detection reagent.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.