Anti-HIV1 Reverse Transcriptase 抗体 [AS35] (Biotin) (ab16162)

製品の概要

  • 製品名
    Anti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin)
    HIV1 Reverse Transcriptase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AS35] to HIV1 Reverse Transcriptase (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: ELISA, IPmore details
  • 免疫原

    Recombinant HIV1 Reverse Transcriptase

  • エピトープ
    Recognizes an epitope on HIV-RT that is different from AS34 epitope.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.08% Sodium Azide
    Constituents: Ca2+ & Mg2+ free Dulbecco's PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Antibody Purity > 95 % by SDS PAGE
  • ポリ/モノ
    モノクローナル
  • クローン名
    AS35
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
IP
  • 追加情報
    ELISA: This antibody has been used as solution phase ab at 2 µg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT.
    IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
    • 別名
      • HIV 1 Reverse transcriptase antibody
      • HIV1 RT antibody
      • Human Immunodeficiency virus 1 antibody
      • p51 RT antibody
      • p66 RT antibody
      • Reverse transcriptase p51 subunit antibody
      • Reverse transcriptase p66 subunit antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol 70:266-74 (1980). Read more (PubMed: 7421592) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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