製品の概要

  • 製品名Anti-HIV1 p55 + p24 + p17 antibody
  • 製品の詳細
    Rabbit polyclonal to HIV1 p55 + p24 + p17
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    Reacts with HIV1.
  • 免疫原

    Recombinant protein p55 over-expressed in E.coli using a plasmid carrying the Gag p55 coding region of HIV1 virus.

  • ポジティブ・コントロール
    • Extract of MT4 cells infected with HIV1 (LAI strain).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 17,24,55 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1.
  • 別名
    • CA antibody
    • Capsid protein p24 antibody
    • Gag antibody
    • Gag polyprotein antibody
    • HIV1 matrix protein p17 antibody
    • HIV1 Pr55Gag antibody
    • Human immunodeficiency virus 1 p17 antibody
    • Human immunodeficiency virus 1 p55 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    • MA antibody
    • Matrix protein antibody
    • Matrix protein p17 antibody
    • Pr55 antibody
    • Pr55Gag antibody
    see all

Anti-HIV1 p55 + p24 + p17 antibody 画像

  • All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution

    Lane 1 : Extract of MT4 cells
    Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)


    Observed band size : 17,24,55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

Anti-HIV1 p55 + p24 + p17 antibody (ab63917) 使用論文

This product has been referenced in:
  • Valera MS  et al. The HDAC6/APOBEC3G complex regulates HIV-1 infectiveness by inducing Vif autophagic degradation. Retrovirology 12:53 (2015). Read more (PubMed: 26105074) »
  • Trabalza A  et al. Enhanced central nervous system transduction with lentiviral vectors pseudotyped with RVG/HIV-1gp41 chimeric envelope glycoproteins. J Virol 88:2877-90 (2014). Read more (PubMed: 24371049) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20000 cells
Specification HeLa
Treatment Transfected with plasmids expressing HIV gag
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

David Carpentier

Verified customer

投稿 Jan 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"