The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Use at an assay dependent dilution.
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
Capsid protein p24 antibody
HIV-1 Gag p24 antibody
Human immunodeficiency virus 1 antibody
Human immunodeficiency virus type 1 p24 antibody
Western blot - HIV1 p24 antibody (ab63914)
All lanes : Anti-HIV1 p24 antibody (ab63914) at 1/2500 dilution
Lane 1 : Extract of MT4 cells Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Predicted band size: 24 kDa Observed band size: 24 kDa
Bands also detected at 41 and 55 kDa representing HIV p24 precursor proteins p41 and p55.