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The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody is useful as solid phase for capture of p24 in an antigen ELISA format
Our Abpromise guarantee covers the use of ab9072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|WB||Use a concentration of 1 - 10 µg/ml.|
|Radioimmunoprecipitation||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/5.4A] to HIV1 p24 (ab9071).|