製品の概要
-
製品名Anti-HIV1 Nef antibody
HIV1 Nef 一次抗体 製品一覧 -
製品の詳細Guinea pig polyclonal to HIV1 Nef
-
由来種Guinea pig
-
アプリケーション適用あり: WB, ELISAmore details
-
種交差性Reacts with HIV-1.
-
免疫原
Recombinant Nef protein over-expressed in E. coli with a plasmid carrying the Nef coding region of HIV1 virus, subtype B.
-
ポジティブ･コントロール
- Extract of MT4 cells uninfected or infected with HIV1 (LAI strain).
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
バッファーPreservative: 0.09% Sodium Azide
Constituents: Whole serum -
精製度Whole antiserum
-
特記事項（精製）ab93919 is highly purified by several steps of chromatography.
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab63919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 24 kDa. | |
| ELISA | Use at an assay dependent dilution. |
ターゲット情報
-
関連性Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
-
別名
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
画像
-
Western blot - Anti-HIV1 Nef antibody (ab63919)All lanes : Anti-HIV1 Nef antibody (ab63919) at 1/1000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)
Predicted band size: 24 kDa
Observed band size: 27 kDa (why is the actual band size different from the predicted?)
プロトコール
参考文献
ab63919 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"