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Bacterially expressed, hexahistidine amino-terminal tagged HIV1 integrase (IN) protein (clade B, HXB-3 isolate)
Our Abpromise guarantee covers the use of ab66645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 32 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
ab66645 staining HIV1 integrase in transfected 293T by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody (1/2000).