Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) (ab115125)


  • 製品名
    Histone H4 Total Acetylation Detection Fast Kit (Colorimetric)
    Histone H4 acetylation キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • 検出感度
    2 ng/well
  • 検出範囲
    5 ng/well - 2000 ng/well
  • 全工程の試験時間
    2h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.

    Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) enables the user to colorimetrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • アプリケーション
    適用あり: Functional Studiesmore details



    Our Abpromise guarantee covers the use of ab115125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.


    Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) (ab115125) 使用論文

    This product has been referenced in:
    • Kocic G  et al. Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease. Ren Fail 36:1078-82 (2014). Functional Studies ; Human . Read more (PubMed: 24845033) »

    See 1 Publication for this product

    Product Wall

    The detection antibody in ab115124 kit is labeled with signal reporter already. But H4 antibody included in the kit ab115125 is not and needs to be labeled by signal reporter and enhanced with enhancer during the assay.

    1. Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

    2. The minimum amount of histone protein required as input for these assays are 50 ng (both for the colorimetric and fluorometric kits...

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    Thank you for contacting us about this exciting idea. Unfortunately our abreview program does not include kits so I cannot offer free or reduced price for this application. I encourage you to please share your findings, good or bad, with us and the...

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