製品の概要

  • 製品名
    Anti-Histone H4 (mono methyl R3) antibody
    Histone H4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H4 (mono methyl R3)
  • 特異性
    ab17339 detects a 14 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab17339 binds strongly to the Histone H4 mono methyl R3 peptide.
  • アプリケーション
    適用あり: WB, Dot blot, ELISA, IHC-P, ICC/IF, PepArrmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide within Human Histone H4 aa 1-100 (N terminal) (mono methyl R3) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab17770)

  • ポジティブ・コントロール
    • WB: Calf thymus histone preparation, HeLa whole cell extract and HL6O lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
ICC/IF Use a concentration of 1 µg/ml.
PepArr Use a concentration of 0.2 - 0.02 µg/ml.

ターゲット情報

画像

  • Anti-Histone H4 (mono methyl R3) antibody (ab17339) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • All batches of ab17339 are tested in Peptide Array against peptides to different Histone H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - mono methyl R3 peptide (ab17770), indicating that this antibody specifically recognises the Histone H4 - mono methyl R3 modification.

    ab17770 - Histone H4 - mono methyl R3

    ab15821 - Histone H4 - unmodified

  • Image courtesy of Human Protein Atlas

    ab17339 staining histone H4 mono methyl R3 in human testis, showing a distinct and strong staining pattern in ductus seminiferus an leydig cells. Paraffin embedded human skin tissue was incubated with ab17339 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab17339 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • ICC/IF image of ab17339 stained human HepG2 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17339, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

参考文献

This product has been referenced in:
  • Fan L  et al. PADI4 Epigenetically Suppresses p21 Transcription and Inhibits Cell Apoptosis in Fibroblast-like Synoviocytes from Rheumatoid Arthritis Patients. Int J Biol Sci 13:358-366 (2017). WB ; Human . Read more (PubMed: 28367100) »
  • Lozano-Amado D  et al. Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica. Parasit Vectors 9:19 (2016). WB, IF . Read more (PubMed: 26767976) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Keratinocytes)
Loading amount
20 µg
Specification
Keratinocytes
Gel Running Conditions
Reduced Denaturing (18)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

投稿 Mar 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary cells)
Loading amount
20 µg
Specification
Primary cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Mar 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録