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Synthetic peptide within Human Histone H4 aa 1-100 (mono methyl K20). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab16974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H4 (mono methyl K20) peptide (ab17043).|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA using ab16974 at antibody concentrations between 1/100 and 1/10000.
The orange line indicates binding to the Histone H4 peptide - mono methyl K20 (ab17043).
Binding to the following peptides was either not seen or was very weak:
Histone H4 peptide - tri methyl K20 (ab17567) - green line,
Histone H4 peptide - di methyl K20 (ab14964) - blue line,
Histone H4 peptide - unmodified (ab2622) - red line.
This indicates the specificity of ab16794 for mono methyl K20 of Histone H4.
ab16974 has not yet been referenced specifically in any publications.