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Synthetic peptide within Human Histone H4 aa 1-100 (mono methyl K20). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab16974 in the following tested applications.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H4 (mono methyl K20) peptide (ab17043).|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA using ab16974 at antibody concentrations between 1/100 and 1/10000.
The orange line indicates binding to the Histone H4 peptide - mono methyl K20 (ab17043).
Binding to the following peptides was either not seen or was very weak:
Histone H4 peptide - tri methyl K20 (ab17567) - green line,
Histone H4 peptide - di methyl K20 (ab14964) - blue line,
Histone H4 peptide - unmodified (ab2622) - red line.
This indicates the specificity of ab16794 for mono methyl K20 of Histone H4.
ab16974 has not yet been referenced specifically in any publications.