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Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 6-55.
Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805) around the acetylation site of lysine 8 (G-G-KA-G-L).
Database link: P62805
Our Abpromise guarantee covers the use of ab61254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab61254 staining Histone H4 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab61254 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H4 staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.
ab61254 has not yet been referenced specifically in any publications.