製品の概要

  • 製品名Anti-Histone H4 antibody
    Histone H4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H4
  • アプリケーション適用あり: ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 6-55. Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805) around the acetylation site of lysine 8 (G-G-KA-G-L).
    Database link: P62805

  • ポジティブ・コントロール
    • Human breast carcinoma tissue. ICC/IF: HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

Anti-Histone H4 antibody 画像

  • ab61254 staining Histone H4 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab61254 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H4 staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.

  • ab61254 at 1/50 - 1/100 dilution staining Histone H4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-Histone H4 antibody (ab61254) 使用論文

ab61254 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab61254.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"