Histone H4 Acetylation Assay Kit (ab115103)
Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Microplate reader
- Assay time: 5 hr
- Sample type: Adherent cells, Suspension cells, Tissue
製品の概要
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製品名
Histone H4 Acetylation Assay Kit
Histone H4 acetylation キット 製品一覧 -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Cell-based (quantitative) -
全工程の試験時間
5h 00m -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals -
製品の概要
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.
Histone H4 Acetylation Assay Kit (ab115103) allows the user to measure global histone H4 acetylation at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Lysis Buffer 1 x 5ml 1 x 10ml 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Acetylated Histone H4 Control (60 µg/mL) 1 x 10µl 1 x 20µl Antibody Buffer 1 x 6ml 1 x 12ml Blocking Buffer 1 x 10ml 1 x 20ml Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Detection Antibody, 400 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 5ml 1 x 10ml Extraction Buffer 1 x 8ml 1 x 16ml Histone Buffer 1 x 0.5ml 1 x 1ml Stop Solution 1 x 3ml 1 x 6ml -
研究分野
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115103 は論文での使用が確認できていません。