Anti-Histone H4 (acetyl K8) 抗体 [EP1002Y] - ChIP Grade (ab45166)

製品の概要

  • 製品名Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade
    Histone H4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1002Y] to Histone H4 (acetyl K8) - ChIP Grade
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IP, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H4 aa 1-100 (N terminal) (acetyl K8). The exact sequence is proprietary.
    Database link: P62805

  • ポジティブ・コントロール
    • WB- HeLa whole cell lysate +TSA, C6 cell lysate, C6 cell + TSA lysate, NIH/3T3 +TSA whole cell lysate IHC-P: Human normal colon FFPE tissue sections, Mouse kidney paraffin-embedded tissue sections, Rat kidney paraffin-embedded tissue sections. ICC/IF- C6 + TSA lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Altertanive versions available : 

    Anti-Histone H4 (acetyl K8) antibody (Alexa Fluor® 488) [EP1002Y] (ab206046
    Anti-Histone H4 (acetyl K8) antibody (Alexa Fluor® 647) [EP1002Y] (ab206047)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/250 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/150 - 1/500.
IP 1/20 - 1/50.
ChIP Use at an assay dependent concentration. PubMed: 22249179

ターゲット情報

  • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性Belongs to the histone H4 family.
  • 翻訳後修飾Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
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    • dJ160A22.1 antibody
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    • H4 histone family, member C antibody
    • H4 histone family, member D antibody
    • H4 histone family, member E antibody
    • H4 histone family, member G antibody
    • H4 histone family, member H antibody
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    • H4 histone family, member J antibody
    • H4 histone family, member K antibody
    • H4 histone family, member M antibody
    • H4 histone family, member N antibody
    • H4 histone, family 2 antibody
    • H4/A antibody
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    • H4/E antibody
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    • H4/H antibody
    • H4/I antibody
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    • H4/K antibody
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    • H4/N antibody
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    • H4F2 antibody
    • H4F2iii antibody
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    • H4FJ antibody
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    • HIST1 cluster, H4A antibody
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    • HIST1 cluster, H4D antibody
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    • Hist4 cluster, H4 antibody
    • Hist4h4 antibody
    • histone 1, H4a antibody
    • histone 1, H4c antibody
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    • histone 1, H4f antibody
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    • histone cluster 1, H4a antibody
    • histone cluster 1, H4b antibody
    • histone cluster 1, H4c antibody
    • histone cluster 1, H4d antibody
    • histone cluster 1, H4e antibody
    • histone cluster 1, H4f antibody
    • histone cluster 1, H4h antibody
    • histone cluster 1, H4i antibody
    • histone cluster 1, H4j antibody
    • histone cluster 1, H4k antibody
    • histone cluster 1, H4l antibody
    • histone cluster 2, H4a antibody
    • histone cluster 2, H4b antibody
    • histone cluster 4, H4 antibody
    • Histone family, member A antibody
    • Histone family, member B antibody
    • Histone family, member D antibody
    • Histone family, member H antibody
    • Histone family, member I antibody
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    • Histone gene cluster 1, H4 antibody
    • Histone gene cluster 1, H4D antibody
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Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade 画像

  • All lanes : Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (ab45166) at 1/5000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma) treated with Trichostatin A whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Blocking and diluting buffer 5% NFDM/TBST
  • Immunocytochemistry/immunofluorescence staining of 4% paraformaldehyde fixed; 0.1% triton X 100 permeabilized C6 (rat glioma) cells (non-treated-top panels) and (C6 + TSA(500ng/ml, 4hr)-middle panels) with purified ab45166 at dilution of 1/150. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right and middle right hand panels. The negative controls are shown in the bottom two panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

  • Immunohistochemical staining of paraffin-embedded mouse kidney sections labelling Histone H4 (acetyl K8) with purified ab45166 at dilution of 1:2500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • ab45166 (purified) at 1/20 immunoprecipitating Histone H4 (acetyl K8) in HeLa treated with Trichostatin A whole cell lysate.

    Lane 1 (input): HeLa treated with Trichostatin A whole cell lysate (10µg)

    Lane 2 (+): ab45166 + HeLa treated with Trichostatin A whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45166 in HeLa treated with Trichostatin A whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (ab45166) at 1/5000 dilution

    Lane 1 : Untreated C6 (rat glioma) whole cell lysate
    Lane 2 : C6 (rat glioma) treated with Trichostatin A whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Blocking and diluting buffer 5% NFDM/TBST
  • Immunohistochemical analysis of formalin fixed paraffin embedded human colon tissue sections labelling Histone H4 (acetyl K8) with unpurified ab45166 at dilution of 1/200.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (ab45166) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo) treated with Trichostatin A whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Blocking and diluting buffer 5% NFDM/TBST
  • Immunohistochemical staining of paraffin-embedded rat kidney sections labelling Histone H4 (acetyl K8) with purified ab45166 at dilution of 1:2500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemical staining of paraffin-embedded human colon sections labelling Histone H4 (acetyl K8) with purified ab45166 at dilution of 1:2500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (ab45166) 使用論文

This product has been referenced in:
  • Kim JY  et al. Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo. J Biol Chem 289:27065-79 (2014). ChIP . Read more (PubMed: 25074928) »
  • Liu Y  et al. BRMS1 suppresses lung cancer metastases through an E3 ligase function on histone acetyltransferase p300. Cancer Res 73:1308-17 (2013). Read more (PubMed: 23269275) »

See all 4 Publications for this product

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