Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) 抗体 (ab10807)

製品の概要

  • 製品名
    Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody
    Histone H4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H4 (acetyl K5 + K8 + K12 + K16)
  • 特異性
    All known acetylated isoforms of histone H4.
  • アプリケーション
    適用あり: ELISA, WB, ICC/IF, IPmore details
  • 種交差性
    Mammals, Amphibia, Drosophila, Yeast & some plants.
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4 aa 1-18 (acetyl K5 + K8 + K12 + K16) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    (N)SGRG(Ac)KGG(Ac)KGLG(Ac)KGGA(Ac)KRH(YCC)


    Database link: P62805

  • 特記事項


    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.are transcribed throughout the cell cycle, there is a 3- to 5-fold increase in transcription during early S phase.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.are transcribed throughout the cell cycle, there is a 3- to 5-fold increase in transcription during early S phase.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/400.
WB 1/800. Predicted molecular weight: 12.4 kDa.
ICC/IF 1/200.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性
    Belongs to the histone H4 family.
  • 翻訳後修飾
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • 細胞内局在
    Nucleus. Chromosome.
  • Information by UniProt
  • 別名
    • Histone gene cluster 1, H4A antibody
    • Histone gene cluster 2, H4 antibody
    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4 histone family, member A antibody
    • H4 histone family, member B antibody
    • H4 histone family, member C antibody
    • H4 histone family, member D antibody
    • H4 histone family, member E antibody
    • H4 histone family, member G antibody
    • H4 histone family, member H antibody
    • H4 histone family, member I antibody
    • H4 histone family, member J antibody
    • H4 histone family, member K antibody
    • H4 histone family, member M antibody
    • H4 histone family, member N antibody
    • H4 histone, family 2 antibody
    • H4/A antibody
    • H4/B antibody
    • H4/C antibody
    • H4/D antibody
    • H4/E antibody
    • H4/G antibody
    • H4/H antibody
    • H4/I antibody
    • H4/J antibody
    • H4/K antibody
    • H4/M antibody
    • H4/N antibody
    • H4/O antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • HIST1 cluster, H4A antibody
    • HIST1 cluster, H4B antibody
    • HIST1 cluster, H4D antibody
    • HIST2H4 antibody
    • Hist4 cluster, H4 antibody
    • Hist4h4 antibody
    • histone 1, H4a antibody
    • histone 1, H4c antibody
    • histone 1, H4d antibody
    • histone 1, H4f antibody
    • histone 1, H4h antibody
    • histone 1, H4i antibody
    • histone 1, H4j antibody
    • histone 1, H4k antibody
    • histone 1, H4l antibody
    • histone 2, H4a antibody
    • histone 2, H4b antibody
    • Histone 4 family, member M antibody
    • histone 4, H4 antibody
    • histone cluster 1, H4 antibody
    • histone cluster 1, H4a antibody
    • histone cluster 1, H4b antibody
    • histone cluster 1, H4c antibody
    • histone cluster 1, H4d antibody
    • histone cluster 1, H4e antibody
    • histone cluster 1, H4f antibody
    • histone cluster 1, H4h antibody
    • histone cluster 1, H4i antibody
    • histone cluster 1, H4j antibody
    • histone cluster 1, H4k antibody
    • histone cluster 1, H4l antibody
    • histone cluster 2, H4a antibody
    • histone cluster 2, H4b antibody
    • histone cluster 4, H4 antibody
    • Histone family, member A antibody
    • Histone family, member B antibody
    • Histone family, member D antibody
    • Histone family, member H antibody
    • Histone family, member I antibody
    • Histone family, member L antibody
    • Histone gene cluster 1, H4 antibody
    • Histone gene cluster 1, H4D antibody
    • Histone gene cluster 1, H4E antibody
    • Histone gene cluster 1, H4K antibody
    • Histone gene cluster 4, H4 antibody
    • Histone gene cluster 4, H4 histone antibody
    • Histone H4 antibody
    • histone IV, family 2 antibody
    see all

Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody (ab10807) 使用論文

ab10807 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Plasmodium falciparum Cell lysate - nuclear (Plasmodium falciparum 3D7 strain)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
5 µg
Specification
Plasmodium falciparum 3D7 strain
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Nanika Coetzee

Verified customer

投稿 May 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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