Anti-Histone H4 (acetyl K12) 抗体 - ChIP Grade (ab46983)

製品の概要

  • 製品名Anti-Histone H4 (acetyl K12) antibody - ChIP Grade
    Histone H4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade
  • 特異性Ab46983 recognizes Histone H4 acetylated at lysine 12. Serum did not react with non-modified Histone H4 peptide or peptides acetylated at lysines 5, 8 or 16.
  • アプリケーション適用あり: Dot blot, WB, ICC/IF, ChIP, IPmore details
  • 種交差性
    交差種: Mouse, Human, Saccharomyces cerevisiae, Xenopus laevis
    交差が予測される動物種: a wide range of other species
  • 免疫原

    This information is considered to be commercially sensitive.

  • ポジティブ・コントロール
    • IF: HeLa cells WB: Acid extract of HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 11 kDa.
ICC/IF 1/500 - 1/2000.
ChIP Use at an assay dependent concentration. 2 - 8 ul per IP
IP Use at an assay dependent concentration.

ターゲット情報

Anti-Histone H4 (acetyl K12) antibody - ChIP Grade 画像

  • Staining of HeLa cells with ab46983 at a dilution of 1/500.

  • ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).

  • ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

  • All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution

    Lane 1 : Acid extract of HeLa cells without treatment.
    Lane 2 : Acid extract of HeLa cells treated with sodium butyrate.


    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ab46983 in dot blot (top panel), acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1000. The amount of peptide (picomoles) spotted is shown next to each row.

    Lane 1: Histone H4 acetyl-Lys5 peptide.
    Lane 2: Unmodified Lys5 peptide.
    Lane 3: Acetyl-Lys8 peptide.
    Lane 4: Unmodified Lys8 peptide.
    Lane 5: Acetyl-Lys12 peptide.
    Lane 6: Unmodified Lys12 peptide.
    Lane 7: Acetyl-Lys16 peptide.
    Lane 8: Unmodified Lys16 peptide.

    ab46983 in WB (bottom panel)at 1/2000 with whole-cell extracts (35 ug/lane)from wildtype yeast or yeast containing a mutated histone H4 gene.
    Lane 1: Wild-type H4.
    Lane 2: H4 N-terminal tail deleted.
    Lane 3: Histone H4 K5 to R.
    Lane 4: H4 with K8 to R.
    Lane 5: H4 with K12 to R.
    Lane 6: H4 K16 to R.
    Lane 7: H4 K5, 8 and 12 to R.

Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) 使用論文

This product has been referenced in:
  • Sun J  et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun 6:8856 (2015). IHC (PFA fixed) ; Drosophila melanogaster . Read more (PubMed: 26581759) »
  • Intlekofer KA  et al. Exercise and Sodium Butyrate Transform a Subthreshold Learning Event into Long-Term Memory via a Brain-Derived Neurotrophic factor-Dependent Mechanism. Neuropsychopharmacology N/A:N/A (2013). Mouse . Read more (PubMed: 23615664) »

See all 8 Publications for this product

Product Wall

Application Flow Cytometry
Sample Mouse Cell (Thymus)
Permeabilization Yes - FIX & PERM® Cell Fixation & Cell Permeabilization Kit
Gating Strategy Thymocytes (CD45+ Epcam- cells)
Specification Thymus
Fixation FIX & PERM® Cell Fixation & Cell Permeabilization Kit
Username

Abcam user community

Verified customer

投稿 May 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 2 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Xenopus laevis Tissue lysate - whole (Xenopus tectum)
Specification Xenopus tectum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. WANHUA SHEN

Verified customer

投稿 Aug 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Fibroblast)
Specification Fibroblast
Fixative Formaldehyde
Permeabilization Yes - 0.05% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - nuclear (Mouse Hippocampus)
Loading amount 10 µg
Specification Mouse Hippocampus
Gel Running Conditions Reduced Denaturing (4-15% gradient gel)
Blocking step Blocking solution from Roti block as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Ms. Gonca Bayraktar

Verified customer

投稿 Jan 26 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"