Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. In particular, H3(try methyl K9) mediates heterochromatin formation by forming a binding site for HP and is a stable heterochromatin mark which promotes gene silencing.is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing.
Histone H3 (tri-methyl K9) Quantification Kit (Fluorometric) (ab115065) allows the user to specifically measure global tri-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
内容 | ラベル | 48 tests | 96 tests |
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10X Wash Buffer | 1 x 10ml | 1 x 20ml | |
8-Well Sample Strips (with Frame) | 4 units | 9 units | |
8-Well Standard Control Strips | Green Ringed | 2 units | 3 units |
Antibody Buffer | 1 x 6ml | 1 x 12ml | |
Detection Antibody, 1 mg/ml | 1 x 5µl | 1 x 10µl | |
Fluoro Developer | 1 x 12µl | 1 x 24µl | |
Fluoro Dilution | 1 x 4ml | 1 x 8ml | |
Fluoro Enhancer | 1 x 12µl | 1 x 24µl | |
Standard Control (100 µg/ml) | 1 x 10µl | 1 x 20µl |
Our Abpromise guarantee covers the use of ab115065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Functional Studies | Use at an assay dependent concentration. |
ab115065 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"