Histone H3 (tri-methyl K9) Quantification Kit (Fluorometric) (ab115065)
製品の概要
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製品名Histone H3 (tri-methyl K9) Quantification Kit (Fluorometric)
Histone H3 (K9 methylation) キット 製品一覧 -
サンプルの種類Tissue, Adherent cells, Suspension cells
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アッセイタイプQuantitative
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検出感度>= 0.4 ng/well
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検出範囲5 ng/well - 2000 ng/well
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全工程の試験時間2h 30m
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種交差性交差種: Mouse, Human
交差が予測される動物種: Mammal
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製品の概要
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. In particular, H3(try methyl K9) mediates heterochromatin formation by forming a binding site for HP and is a stable heterochromatin mark which promotes gene silencing.is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing.
Histone H3 (tri-methyl K9) Quantification Kit (Fluorometric) (ab115065) allows the user to specifically measure global tri-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. -
アプリケーション適用あり: Functional Studiesmore details
製品の特性
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保存方法Please refer to protocols.
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内容 ラベル 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Detection Antibody, 1 mg/ml 1 x 5µl 1 x 10µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl -
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab115065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
画像
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Representative Assay DataMeasurement of Histone H3 (tri-methyl K9) in total histone extracts.
プロトコール
参考文献
ab115065 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"