Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric) (ab115072)

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製品の概要

  • 製品名
    Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)
    Histone H3 (K27 methylation) キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプ
    Quantitative
  • 検出感度
    >= 2 ng/well
  • 検出範囲
    20 ng/well - 5000 ng/well
  • 全工程の試験時間
    2h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, H3(try methyl K27) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.

    Histone H3K27me3 is quantified colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

プロトコール

参考文献

ab115072 has not yet been referenced specifically in any publications.

ab115072 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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4 reviews or Q&A

10
Votes: Highest First

The credit was about ******* and this time, he has ******.
These kits were not satisfied again, he already purchased other vendor's products and doesn't need to use ab115070, ab115072 any more.
Please arrange credit note for our customer.

Sorry for the confusion.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: *****

As usual if you have any fu...

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Please arrange credit note for our customer.

A credit note has already been issued on the DD MM YYYY for these two kits. The reference of the credit note is ********.

In my previous email I said : "Would your customer agree to use their credit note to buy and try the kits for the 3...

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As you know our customer is is havinga problem using you ab115070, ab115072.
He recently received new kits and tested again, but he could not any results eventhoug at standard.
So today I met him and checked his protocols, and there was no pr...

Read More

Please find below the last email I received from the lab where the kits are produced :

"We are sorry to hear about it and not clear what caused it not work.
The procedure seems fine.
We did not receive any other complaint for t...

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As you know our customer is is havinga problem using you ab115070, ab115072.
He recently received new kits and tested again, but he could not any results eventhoug at standard.
So today I met him and checked his protocols, and there was no pr...

Read More

Thank you for contacting us.

Thank you also for the detailed information about the use of the kits ab115070 and ab115072.

I do believe there is a problem with those kits, so please send our sincere apologies to the customer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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