Histone H3 Total Quantification Kit (Fluorometric) (ab115092)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 2 ng/well
製品の概要
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製品名
Histone H3 Total Quantification Kit (Fluorometric)
Histone H3 キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
検出感度
2 ng/well -
検出範囲
10 ng/well - 1000 ng/well -
全工程の試験時間
2h 30m -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Mammals -
製品の概要
Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.
Histone H3 Total Quantification Kit (Fluorometric) (ab115092) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 ラベル 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8 well Sample Strips (with frame) 4 units 9 units 8 well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl -
研究分野
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関連性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
細胞内局在
Nucleus. Chromosome. -
別名
- H3f3a
- H3FA
- H3FB
see all -
参照データベース
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
see all
関連製品
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Related Products
- Histone H3 (di-methyl K4) Quantification Kit (Colorimetric) (ab115052)
- Histone H3 (di-methyl K4) Quantification Kit (Fluorometric) (ab115053)
- Histone H3 (mono-methyl K4) Quantification Kit (Colorimetric) (ab115054)
- Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric) (ab115055)
- Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056)
- Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057)
- Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric) (ab115058)
- Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)
- Histone H3 Total Quantification Kit (Colorimetric) (ab115091)
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115092 は論文での使用が確認できていません。