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Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. Detection in the change of histone H3 (phospho S10) associated with the cell cycle, apoptosis, inhibitor or activator treatment would provide useful information for better understanding the pathological process of some diseases.
Abcam's Histone H3 (phospho S10) Assay Kit (In Situ) (ab115126) allows the user to measure histone H3 (phospho S10) in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
|10X Wash Buffer||1 x 30ml|
|30% H2O2 Solution||1 x 0.5ml|
|8-Well Control Strips||1 x 2 units|
|Antibody Buffer||1 x 15ml|
|Blocking Buffer||1 x 20ml|
|Capture Antibody, 1mg/mL||1 x 6µl|
|Detection Antibody, 200 µg/mL||1 x 20µl|
|Developing Solution||1 x 12ml|
|Permeabilizing Buffer||1 x 30ml|
|Phospho H3Ser10 Control, 20 µg/mL||1 x 15µl|
|Stop Solution||1 x 6ml|
Our Abpromise guarantee covers the use of ab115126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115126 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"