Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060)

製品の概要

  • 製品名Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric)
    Histone H3 (K9 methylation) キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 全工程の試験時間
    2h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases mediates heterochromatin formation by forming a binding site for HP1. H3 (mono-methyl K9) is enriched in certain euchromatin domains which have been postulated to be transcriptionally silent.

    Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060) allows the user to specifically measure global mono-methylation of histone H3K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab115060 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) 画像

    • Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of H3K9me was measured using ab115060.

    プロトコール

    Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060) 使用論文

    ab115060 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"