Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069)

製品の概要

  • 製品名Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric)
    Histone H3 (K27 methylation) キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 検出感度
    0.5 ng/well
  • 検出範囲
    20 ng/well - 5000 ng/well
  • 全工程の試験時間
    2h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (mono-methyl K27), a modification enriched at pericentromeric heterochromatin, was observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.

    Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069) allows the user to specifically measure global mono-methylation of histone H3K27 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

     

  • 特記事項

     

     

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab115069 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) 画像

    • ab115069 used to measure the amount of mono-methyl-H3K27. Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476).

    プロトコール

    Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069) 使用論文

    ab115069 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115069.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"