Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069)

製品の概要

  • 製品名
    Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric)
    Histone H3 (K27 methylation) キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプ
    Quantitative
  • 検出感度
    0.5 ng/well
  • 検出範囲
    20 ng/well - 5000 ng/well
  • 全工程の試験時間
    2h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (mono-methyl K27), a modification enriched at pericentromeric heterochromatin, was observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.

    Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069) allows the user to specifically measure global mono-methylation of histone H3K27 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

     

  • 特記事項

     

     

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab115069 used to measure the amount of mono-methyl-H3K27. Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476).

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参考文献

ab115069 has not yet been referenced specifically in any publications.

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