製品の概要

  • 製品名Histone H3 (methyl K9) Assay Kit (In Situ)
    Histone H3 (K9 methylation) キット 製品一覧
  • サンプルの種類
    Adherent cells
  • アッセイタイプCell-based (quantitative)
  • 全工程の試験時間
    3h 00m
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: all Mammals
  • 製品の概要

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.

    Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047) allows the user to specifically measure histone H3K9me in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 保存方法Please refer to protocols.
  • 内容 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 15ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 100 µg/mL 1 x 60µl 1 x 120µl
    Detection Antibody, 400 µg/mL 10 x 20µl 1 x 40µl
    Developing Solution 1 x 12ml 1 x 24ml
    Methylated H3K9 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab115047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    プロトコール

    Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047) 使用論文

    ab115047 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"