Histone H3 (K9 methylation) Panel (H3, mono methyl K9, di methyl K9, tri methyl K9) (ab113754)

製品の概要

  • 製品名Histone H3 (K9 methylation) Panel (H3, mono methyl K9, di methyl K9, tri methyl K9)
  • 製品の概要

    ab113754 is a Histone H3 (K9 methylation) Panel designed for the validation and characterization of the methylation state of Histone H3 on K9. Methylation of the canonical core histones can contribute to the formation of transcriptionally active and inactive chromatin in response to various signalling pathways and is a central modification for regulating epigenetic transitions in chromatin. Histone H3 methylation on K9 is a repressive modification, and H3 K9 tri-methylation has been shown to establish binding sites for heterochromatin protein 1.

製品の特性

  • 別名
    • FLJ92264
    • H3 histone
    • H3 histone family member E pseudogene
    • H3 histone family, member A
    • H31_HUMAN
    • H3F3
    • HIST1H3J
    • HIST3H3
    • Histone H 3
    • Histone H3 (methylated)
    • Histone H3 - unmodified
    • Histone H3 3 pseudogene
    • Histone H3.1
    • Histone H3/a
    • Histone H3/b
    • Histone H3/c
    • Histone H3/d
    • Histone H3/f
    • Histone H3/h
    • Histone H3/i
    • Histone H3/j
    • Histone H3/k
    • Histone H3/l
    see all

Histone H3 (K9 methylation) Panel (H3, mono methyl K9, di methyl K9, tri methyl K9) 画像

  • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1791 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
  • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab9045 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
  • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab8898 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
  • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
  • All lanes : Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab9045) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Human Histone H3 (unmodified ) peptide (ab7228)
    Lane 3 : Human Histone H3 (mono methyl K27) peptide (ab1780)
    Lane 4 : Human Histone H3 (di methyl K27) peptide (ab1781)
    Lane 5 : Human Histone H3 (tri methyl K27) peptide (ab1782)
    Lane 6 : Human Histone H3 (mono methyl K4) peptide (ab1340)
    Lane 7 : Human Histone H3 (mono methyl K9) peptide (ab1771)
    ab9045 shows significantly greater reactivity with mono methyl K9. This can be seen in lane 7, as the addition of ab1771 (mono methyl K9) completely blocks the activity of ab9045. Weaker cross-reactivity is seen against mono methyl K27. This is shown in lane 3, as the addition of ab1780 only partially blocks the activity of ab9045.

Histone H3 (K9 methylation) Panel (H3, mono methyl K9, di methyl K9, tri methyl K9) (ab113754) 使用論文

This product has been referenced in:
  • Nakamura M  et al. Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila. Nat Commun 5:5264 (2014). Read more (PubMed: 25345385) »

See 1 Publication for this product

Product Wall

I have had discussion with laboratory for the smaller sizes of ab9050 and ab9049. Actually they won't be able to treat these abs as a panel and wouldn’t like to split these in 4 units. I am sorry we won't be creating a new panel with ab9050 and ab9049

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"