Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)

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製品の概要

  • 製品名
    Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit
    Histone Methyltransferase キット 製品一覧
  • アッセイタイプ
    Quantitative
  • 全工程の試験時間
    3h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.
    Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3K4). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Functional Studies - Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)
    Functional Studies - Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)
    Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K4 specific histone methyltrasferase activity (SET7/9) was measured.

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参考文献

ab113452 has not yet been referenced specifically in any publications.

ab113452 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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3 reviews or Q&A

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Dear Sir/Madam, Thank you for your quick reply and clarification. I would like to ask for other techical information: 1. What is the usefull concentration range of purified HMT enzyme (H3-K4 specific) for this kit (ab113452)? 2. Can I use the pro...

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Thank you contacting us with these further questions. I have spoken with the lab about a usefull concentration for the purified HMT enzyme. Based on tests, we conclude that the useful concentration range can be from 10 ng to 200 ng/well (30 ul). ...

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Dear Sir/Madam, I would like to have some techical information about your product ab113452 EpiSeeker Histone Methyltransferase H3(K4) Activity Quantification Assay Kit. - Can I use a purified HMT enzyme rather than nuclear extr...

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Thank you for contacting us. I have had a chance to speak with the lab regarding your question. They have told me that the purified HMT enzyme (H3-K4 specific) can be used in this assay kit. I hope this information is helpful to you. Please do ...

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Dear Sir/Madam,
I would like to have some technical information about your product
ab113452 EpiSeeker Histone Methyltransferase H3(K4) Activity
Quantification Assay Kit.
1. Could you indicate what is biotinylated substrate (EHM4) us...

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Thank you for your enquiry regarding ab113452.
1. The biotinylated substrate is unmethylated histone peptide and used for H3-K9 specific histone methyltransferase reaction.
2. For generating a standard curve, first, dilute HK5 HMT Standard wi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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