Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)
製品の概要
- 製品名Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit
Histone Methyltransferase キット 製品一覧 - アッセイタイプQuantitative
- 全工程の試験時間3h 00m
- 種交差性交差種: Mouse, Human
交差が予測される動物種: Mammal
- 製品の概要
Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.
Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3K4). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment. - アプリケーション適用あり: Functional Studiesmore details
製品の特性
- 保存方法Please refer to protocols.
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内容 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Adomet 1 x 25µl 1 x 50µl Biotinylated Substrate, 25 µg/mL 1 x 100µl 1 x 200µl Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Control Enzyme (100 µg/mL) 1 x 10µl 1 x 20µl Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 6ml 1 x 12ml Histone Assay Buffer 1 x 1.5ml 1 x 3ml HMT Standard, 10 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml - 研究分野
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アプリケーション
Our Abpromise guarantee covers the use of ab113452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit 画像
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Functional Studies - Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K4 specific histone methyltrasferase activity (SET7/9) was measured.
プロトコール
Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) 使用論文
ab113452 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"