Histone H3 (di-methyl K4) Quantification Kit (Fluorometric) (ab115053)

製品の概要

  • 製品名
    Histone H3 (di-methyl K4) Quantification Kit (Fluorometric)
    Histone H3 (K4 methylation) キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプ
    Quantitative
  • 全工程の試験時間
    2h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. H3 (di-methyl K4) is associated with transcriptionally permissive euchromatin regions in the genome; in particular, Enrichment of H3 (di-methyl K4) is present across the body of genes, where contributes to mRNA processing.

    Histone H3 (di-methyl K4) Quantification Kit (Fluorometric) (ab115053) allows the user to specifically measure global di-methylation of histone H3K4 fluorometrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

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  • Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of H3K4me2 was measured using ab115053.

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参考文献

ab115053 has not yet been referenced specifically in any publications.

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