Histone H3 Acetylation Assay Kit (ab115102)

製品の概要

  • 製品名
    Histone H3 Acetylation Assay Kit
    Histone H3 acetylation キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプ
    Cell-based (quantitative)
  • 全工程の試験時間
    5h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.

     Histone H3 Acetylation Assay Kit (ab115102) allows the user to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab115102 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    プロトコール

    Histone H3 Acetylation Assay Kit (ab115102) 使用論文

    ab115102 has not yet been referenced specifically in any publications.

    Product Wall

    Histone H3 Acetylation in Zebrafish embryos

    Average Good 4/5 (Ease of Use)
    Abreviews
    Sample Type: Histone from zebrafish embryos at 3hpf.
    The assay procedure was made follow the manufacture´s instructions, using 200 ng/ul of histone extract. The positive control (H3 acetilated control) was diluted 1:1000. For the trial we included the positive and negative control (Histone buffer) and the diluted histone sample.
    The results obtained after measure the absorbance at 450 nm are shown in the image.
    Username

    Abcam user community

    Verified customer

    投稿 Feb 08 2017

    Histone H3 Acetylation in Zebrafish Testicle Cells

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Sample Type: Cells dissociated from zebrafish testicle.
    Extraction of histones: the protocol described in the datasheed did not work well. The difficult was to resuspended the tissue (or cells when dissociated) in the Lysis Buffer. When the tissue was resuspended appeared a mucus material that made impossible to resuspend well the tissue.
    For that reason, we chose another methology (used routinely in our lab): dissociated tissue was subjected to hypotonic lysis buffer (10mM Tris-Cl pH 8.0 1mM KCl 1.5 mM MgCl2 1mM DTT) and the obtained pellet was solubilized in HCl 0.4 N (to obtain the basic proteins). After this incubation step, we proceed with a common precipitation with TCA/Acetone and the final protein pellet was solubilized in molecular biology water. With a starting material of 2·10^8 cel/mL we obtained aproximately 6000 ug/mL of histones.
    Assay procedure: the histones obtained as was described before were diluted to 300 ng/uL and were used as the protocol described. For the trial we included a negative control (water), a positive control (H3 Acetilated Control at 15 ng/uL) and the diluted histone sample.
    Results of absorbance at 450 nm are shown in the image below.
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    Miss. Silvia González Rojo

    Verified customer

    投稿 Jan 26 2017

    Compared to ab115102, ab115124 is much faster and easier to handle with higher sensitivity and better reproducibility, but slightly less specificity. 1-2 ug of histone protein (min 50,000 cells) may be needed for each assay point if using ab115102, whi...

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    Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

    I have discussed your question with some colleagues in the Lab and can confirm that RIPA buffer would not be compatible with the 10X Lysis Buffer.

    If needed, you can purchase the 10X Lysis Buffer component individually from us.

    >The kits are very similar. The main differences are that the ab115102 requires 5 hrs to run and you need to coat the antibody yourself while the kit ab115124 only requires 2.5 hours and the wells come pre-coated.

    Regarding the use of a 60 or 100mm plate - this is used for disecting tissue samples if you are using them, however, once you proceed with the histone extraction, the assay is then performed in the strip wells provided with the kit. The strips can be p...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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