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ART[Acetyl-K]QTAR, corresponding to amino acids 2-9 of Human Histone H3 in which the acetylation site is located at lysine 4.
Our Abpromise guarantee covers the use of ab113672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
IHC image of ab113672 staining Histone H3 (acetyl K4) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113672, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.