Anti-Histone H3 (acetyl K14) 抗体 [EP964Y] - ChIP Grade (ab52946)

製品の概要

  • 製品名Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade
    Histone H3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP964Y] to Histone H3 (acetyl K14) - ChIP Grade
  • 特異性There was no cross-reactivity observed with recombinant H3 or the following modifications Acetyl-K9/pS10, -K18, -K23, and -K27 in dot plot. We have data to indicate that this antibody will not cross react with mouse. The antibody was tested against brain, kidney, spleen and heart lysates.
  • アプリケーション適用あり: IHC - Wholemount, ChIP, WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Rat, Human, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe
  • 免疫原

    A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 of Histone H3

  • ポジティブ・コントロール
    • C6 cell lysates, human adenocarcinoma of uterus tissue and HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Histone H3 (acetyl K14) antibody (Biotin) [EP964Y] - (ab200031)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC - Wholemount Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 19188451
WB 1/2000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/20.
IHC-P 1/100 - 1/250.
ICC/IF 1/250 - 1/500.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 配列類似性Belongs to the histone H3 family.
    • 発生段階Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    • 翻訳後修飾Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
      Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
    • 細胞内局在Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H3.3A antibody
      • HIST1 cluster, H3E antibody
      • H3 histone family, member A antibody
      • H3.1 antibody
      • H3/l antibody
      • H3F3 antibody
      • H3FF antibody
      • H3FJ antibody
      • H3FL antibody
      • Histone gene cluster 1, H3 histone family, member E antibody
      • histone H3.1t antibody
      • Histone H3/o antibody
      • FLJ92264 antibody
      • H 3 antibody
      • H3 antibody
      • H3 histone family, member B antibody
      • H3 histone family, member C antibody
      • H3 histone family, member D antibody
      • H3 histone family, member F antibody
      • H3 histone family, member H antibody
      • H3 histone family, member I antibody
      • H3 histone family, member J antibody
      • H3 histone family, member K antibody
      • H3 histone family, member L antibody
      • H3 histone family, member T antibody
      • H3 histone, family 3A antibody
      • H3/A antibody
      • H3/b antibody
      • H3/c antibody
      • H3/d antibody
      • h3/f antibody
      • H3/h antibody
      • H3/i antibody
      • H3/j antibody
      • H3/k antibody
      • H3/t antibody
      • H31_HUMAN antibody
      • H3F1K antibody
      • H3F3A antibody
      • H3FA antibody
      • H3FB antibody
      • H3FC antibody
      • H3FD antibody
      • H3FH antibody
      • H3FI antibody
      • H3FK antibody
      • HIST1 cluster, H3A antibody
      • HIST1 cluster, H3B antibody
      • HIST1 cluster, H3C antibody
      • HIST1 cluster, H3D antibody
      • HIST1 cluster, H3F antibody
      • HIST1 cluster, H3G antibody
      • HIST1 cluster, H3H antibody
      • HIST1 cluster, H3I antibody
      • HIST1 cluster, H3J antibody
      • HIST1H3A antibody
      • HIST1H3B antibody
      • HIST1H3C antibody
      • HIST1H3D antibody
      • HIST1H3E antibody
      • HIST1H3F antibody
      • HIST1H3G antibody
      • HIST1H3H antibody
      • HIST1H3I antibody
      • HIST1H3J antibody
      • HIST3H3 antibody
      • Histone 1, H3a antibody
      • Histone 1, H3b antibody
      • Histone 1, H3c antibody
      • Histone 1, H3d antibody
      • Histone 1, H3e antibody
      • Histone 1, H3f antibody
      • Histone 1, H3g antibody
      • Histone 1, H3h antibody
      • Histone 1, H3i antibody
      • Histone 3, H3 antibody
      • histone cluster 1 H3 family member a antibody
      • histone cluster 1 H3 family member b antibody
      • histone cluster 1 H3 family member c antibody
      • histone cluster 1 H3 family member d antibody
      • histone cluster 1 H3 family member e antibody
      • histone cluster 1 H3 family member f antibody
      • histone cluster 1 H3 family member g antibody
      • histone cluster 1 H3 family member h antibody
      • histone cluster 1 H3 family member i antibody
      • histone cluster 1 H3 family member j antibody
      • Histone cluster 1, H3a antibody
      • Histone cluster 1, H3b antibody
      • Histone cluster 1, H3c antibody
      • Histone cluster 1, H3d antibody
      • Histone cluster 1, H3e antibody
      • Histone cluster 1, H3f antibody
      • Histone cluster 1, H3g antibody
      • Histone cluster 1, H3i antibody
      • Histone cluster 1, H3j antibody
      • Histone gene cluster 1, H3 histone family, member A antibody
      • Histone gene cluster 1, H3 histone family, member B antibody
      • Histone gene cluster 1, H3 histone family, member C antibody
      • Histone gene cluster 1, H3 histone family, member D antibody
      • Histone gene cluster 1, H3 histone family, member F antibody
      • Histone gene cluster 1, H3 histone family, member G antibody
      • Histone gene cluster 1, H3 histone family, member H antibody
      • Histone gene cluster 1, H3 histone family, member I antibody
      • Histone gene cluster 1, H3 histone family, member J antibody
      • Histone gene cluster 1, H3A antibody
      • Histone gene cluster 1, H3B antibody
      • Histone gene cluster 1, H3C antibody
      • Histone gene cluster 1, H3D antibody
      • Histone gene cluster 1, H3E antibody
      • Histone gene cluster 1, H3F antibody
      • Histone gene cluster 1, H3G antibody
      • Histone gene cluster 1, H3H antibody
      • Histone gene cluster 1, H3I antibody
      • Histone gene cluster 1, H3J antibody
      • Histone H 3 antibody
      • Histone H3.1 antibody
      • Histone H3.2 antibody
      • Histone H3.3 antibody
      • Histone H3/a antibody
      • Histone H3/b antibody
      • Histone H3/c antibody
      • Histone H3/d antibody
      • Histone H3/f antibody
      • Histone H3/h antibody
      • Histone H3/i antibody
      • Histone H3/j antibody
      • Histone H3/k antibody
      • Histone H3/l antibody
      • Histone H3/m antibody
      see all

    Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade 画像

    • Immunocytochemistry/Immunofluorescence analysis of untreated HeLa cells and TSA (500ng/ml, 4h) and treated HeLa cells labelling Histone H3 (acetyl K14) with purified ab52946 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, counterstained with ab150120 AlexaFluor®594 Goat anti-Mouse secondary 1:1000  (2ug/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody(ab150120)

      Negative Control 2: Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)

    • All lanes : Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (ab52946) at 1/2000 dilution

      Lane 1 : C6 cell lysates untreated
      Lane 2 : C6 cell lysates treated with TSA

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • ab52946 at 1/100-1/250 dilution staining Histone H3 (acetyl K14) in human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
    • IHC - Wholemount of Caenorhabditis elegans embryo labelling Histone H3 with ab52946. Sample was incubated with primary antibody (1/200 in TBS + 5% BSA + 0.1% Triton-X 100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/1000) was used as the secondary antibody.

      See Abreview

    • ab52946 immunoprecipitating Histone H3 (acetyl K14) in Drosophila melanogaster embryo whole tissue lysate. 500µg of tissue lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 2 hours at 4°C. For western blotting ab52946 (1/20000) was used to confirm successful immunoprecipation.

      See Abreview

    Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (ab52946) 使用論文

    This product has been referenced in:
    • Zhang ZQ  et al. Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep 6:23912 (2016). WB . Read more (PubMed: 27045575) »
    • de Assuncao TM  et al. New Role for Kruppel-like Factor 14 as a Transcriptional Activator Involved in the Generation of Signaling Lipids. J Biol Chem 289:15798-15809 (2014). ChIP ; Human . Read more (PubMed: 24759103) »

    See all 12 Publications for this product

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Caenorhabditis elegans Tissue lysate - whole (C. elegans whole body)
    Specification C. elegans whole body
    Total protein in input 800 µg
    Username

    Abcam user community

    Verified customer

    投稿 Mar 20 2014

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
    Specification Fruit fly embryo
    Total protein in input 500 µg
    Username

    Abcam user community

    Verified customer

    投稿 Mar 20 2014

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Mouse Cell lysate - nuclear (Nuclear extract of mouse embryonic fibroblasts)
    Specification Nuclear extract of mouse embryonic fibroblasts
    Total protein in input 200 µg
    Username

    Abcam user community

    Verified customer

    投稿 Mar 20 2014

    Application IHC - Wholemount
    Sample Caenorhabditis elegans Embryo (C. elegans embryo)
    Specification C. elegans embryo
    Username

    Abcam user community

    Verified customer

    投稿 Mar 18 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample Mouse Cell (Mouse embryonic fibroblasts)
    Specification Mouse embryonic fibroblasts
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Nov 28 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Apr 18 2012

    Thank you for contacting us. I have been in touch with the lab and can confirm that the antibody has been tested against mouse brain, kidney, spleen and heart in western blot, and no signal was observed. I hope this information helps.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"