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Our Abpromise guarantee covers the use of ab23913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/3000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
Image courtesy of Human Protein Atlas
ab23913 staining Histone H2B (testis specific) in human testis tissue, showing a distinct and strong staining pattern within the seminiferous tubules. Paraffin embedded human testis was incubated with ab3167 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab23913 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org