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RabMAb

Anti-Histone H2B (acetyl K5) 抗体 [EP857Y] - ChIP Grade (ab40886)

製品の概要

  • 製品名
    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade
    Histone H2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
  • 特異性
    There is cross-reactivity with H3K27Ac (Histone H3 acetylated on Lys 27).
  • アプリケーション
    適用あり: WB, IHC - Wholemount, CHIPseq, ChIP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human, Caenorhabditis elegans
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K5).

  • ポジティブ・コントロール
    • TSA-treated A431 cell lysate, human urinary bladder carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC - Wholemount Use at an assay dependent concentration.
CHIPseq Use at an assay dependent concentration.
ChIP Use 6-25 µg for µg of chromatin.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 配列類似性
      Belongs to the histone H2B family.
    • 翻訳後修飾
      Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    • 細胞内局在
      Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H2B 1A antibody
      • H2B antibody
      • H2B histone family antibody
      • H2B2f antibody
      • H2B2F_HUMAN antibody
      • H2Ba antibody
      • H2Bf antibody
      • HIST2H2BF antibody
      • histone H2B antibody
      • histone H2B type 1 antibody
      • Histone H2B type 2-F antibody
      • MGC131639 antibody
      see all

    画像

    • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.    

    • All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/50000 dilution

      Lane 1 : A431 cell lysate - untreated
      Lane 2 : A431 cell lysate - treated with TSA

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa
      Observed band size : 14 kDa
    • ab40886 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
    • ab40886 (1:250) staining A431 cells by immunofluorescence.
    • ab40886 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.
      Detection step: Semiquantitative PCR.
      Negative control: Rabbit IgG.
      Cells treated with active vitamin D3.

      See Abreview

    • IHC - Wholemount of Caenorhabditis elegans larvae labelling Histone H2B (acetyl K5) with ab40886. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/1000), was used as the secondary antibody.

      See Abreview

    参考文献

    This product has been referenced in:
    • Gao J  et al. HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum. Front Cell Neurosci 10:221 (2016). IHC-Fr ; Xenopus laevis . Read more (PubMed: 27729849) »
    • Shearstone JR  et al. Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS One 11:e0153767 (2016). ChIP . Read more (PubMed: 27073918) »

    See all 5 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly larvae)
    Specification
    Fruit fly larvae
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    投稿 Jan 08 2015

    Application
    IHC - Wholemount
    Sample
    Caenorhabditis elegans Embryo (C. elegans larvae)
    Specification
    C. elegans larvae
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    投稿 Dec 30 2013

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (Rat olfactory bulb frozen section)
    Specification
    Rat olfactory bulb frozen section
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
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    投稿 Dec 30 2013

    Application
    Immunocytochemistry
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Human Cultured Cells (Human embryonic stem cells)
    Specification
    Human embryonic stem cells
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
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    Verified customer

    投稿 Dec 27 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ChIP
    Sample
    Mouse Tissue lysate - whole (dorsal skin epidermis)
    Specification
    dorsal skin epidermis
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Semiquantitative PCR
    Negative control
    Rabbit IgG
    Username

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    Verified customer

    投稿 Feb 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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