Anti-Histone H2B (acetyl K5) 抗体 [EP857Y] - ChIP Grade (ab40886)

製品の概要

  • 製品名Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade
    Histone H2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
  • 特異性There is cross-reactivity with H3K27Ac (Histone H3 acetylated on Lys 27).
  • アプリケーション適用あり: WB, IHC - Wholemount, CHIPseq, ChIP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human, Caenorhabditis elegans
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K5).

  • ポジティブ・コントロール
    • TSA-treated A431 cell lysate, human urinary bladder carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC - Wholemount Use at an assay dependent concentration.
CHIPseq Use at an assay dependent concentration.
ChIP Use 6-25 µg for µg of chromatin.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 配列類似性Belongs to the histone H2B family.
    • 翻訳後修飾Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    • 細胞内局在Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H2B 1A antibody
      • H2B antibody
      • H2B histone family antibody
      • H2B2f antibody
      • H2B2F_HUMAN antibody
      • H2Ba antibody
      • H2Bf antibody
      • HIST2H2BF antibody
      • histone H2B antibody
      • histone H2B type 1 antibody
      • Histone H2B type 2-F antibody
      • MGC131639 antibody
      see all

    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade 画像

    • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.    

    • All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/50000 dilution

      Lane 1 : A431 cell lysate - untreated
      Lane 2 : A431 cell lysate - treated with TSA

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa
      Observed band size : 14 kDa
    • ab40886 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
    • ab40886 (1:250) staining A431 cells by immunofluorescence.
    • ab40886 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.
      Detection step: Semiquantitative PCR.
      Negative control: Rabbit IgG.
      Cells treated with active vitamin D3.

      See Abreview

    • IHC - Wholemount of Caenorhabditis elegans larvae labelling Histone H2B (acetyl K5) with ab40886. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/1000), was used as the secondary antibody.

      See Abreview

    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) 使用論文

    This product has been referenced in:
    • Wang Z  et al. Combinatorial patterns of histone acetylations and methylations in the human genome. Nat Genet 40:897-903 (2008). CHIPseq . Read more (PubMed: 18552846) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12.5%)
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly larvae)
    Specification Fruit fly larvae
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    投稿 Jan 08 2015

    Application IHC - Wholemount
    Sample Caenorhabditis elegans Embryo (C. elegans larvae)
    Specification C. elegans larvae
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    投稿 Dec 30 2013

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step None
    Sample Rat Tissue sections (Rat olfactory bulb frozen section)
    Specification Rat olfactory bulb frozen section
    Permeabilization Yes - 0.1% Triton X-100
    Fixative Paraformaldehyde
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    投稿 Dec 30 2013

    Application Immunocytochemistry
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample Human Cultured Cells (Human embryonic stem cells)
    Specification Human embryonic stem cells
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
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    投稿 Dec 27 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ChIP
    Sample Mouse Tissue lysate - whole (dorsal skin epidermis)
    Specification dorsal skin epidermis
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step Semiquantitative PCR
    Negative control Rabbit IgG
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    投稿 Feb 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"