Anti-Histone H2B (acetyl K20) 抗体 [EP859Y] - ChIP Grade (ab52988)


  • 製品名Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade
    Histone H2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP859Y] to Histone H2B (acetyl K20) - ChIP Grade
  • 特異性ab52988 only detects Histone H2B acetylated on Lys 20.
  • アプリケーション適用あり: WB, IP, Flow Cyt, IHC-P, ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Histone H2B aa 1-100 (acetyl K20).

  • ポジティブ・コントロール
    • C6 cell lysates (untreated or treated with TSA) and human cervical carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab52988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IP 1/10.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
ChIP Use at an assay dependent concentration. PubMed: 20505338


  • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性Belongs to the histone H2B family.
  • 翻訳後修飾Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
    Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • H2B 1A antibody
    • H2B antibody
    • H2B histone family antibody
    • H2B2f antibody
    • H2B2F_HUMAN antibody
    • H2Ba antibody
    • H2Bf antibody
    • HIST2H2BF antibody
    • histone H2B antibody
    • histone H2B type 1 antibody
    • Histone H2B type 2-F antibody
    • MGC131639 antibody
    see all

Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade 画像

  • All lanes : Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade (ab52988) at 1/50000 dilution

    Lane 1 : C6 cell lysate, untreated
    Lane 2 : C6 cell lysate, treated with TSA

    Lysates/proteins at 10 µg per lane.

    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • ab52988 at 1/250-1/500 dilution staining Histone H2B in human cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.
  • ICC/IF image of ab52988 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52988, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab52988 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52988, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab52988 showing positive staining in Normal tonsil tissue.

  • ab52988 showing positive staining in Ovarian carcinoma tissue.

  • ab52988 showing positive staining in Thyroid gland carcinoma tissue.

  • ab52988 showing positive staining in Breast carcinoma tissue.

  • ab52988 showing positive staining in Colonic adenocarcinoma tissue.

Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade (ab52988) 使用論文

This product has been referenced in:
  • Zhang B  et al. An integrated peptide-antigen microarray on plasmonic gold films for sensitive human antibody profiling. PLoS One 8:e71043 (2013). Read more (PubMed: 23923050) »
  • Price JV  et al. On silico peptide microarrays for high-resolution mapping of antibody epitopes and diverse protein-protein interactions. Nat Med : (2012). Read more (PubMed: 22902875) »

See all 3 Publications for this product

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