Anti-Histone H2A.Z 抗体 [EPR6172] (ab124793)

製品の概要

  • 製品名Anti-Histone H2A.Z antibody [EPR6172]
    Histone H2A.Z 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6172] to Histone H2A.Z
  • 特異性This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2).
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2A.Z aa 100 to the C-terminus (C terminal).

  • ポジティブ・コントロール
    • C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates , Human breast tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR6172
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • 配列類似性Belongs to the histone H2A family.
    • 翻訳後修飾Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • 細胞内局在Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Anti-Histone H2A.Z antibody [EPR6172] 画像

    • All lanes : Anti-Histone H2A.Z antibody [EPR6172] (ab124793) at 1/1000 dilution

      Lane 1 : C6 lysates
      Lane 2 : Raw 264.7 lysates
      Lane 3 : PC-12 lysates
      Lane 4 : NIH/3T3 lysates
      Lane 5 : HeLa lysates
      Lane 6 : HepG2 lysates
      Lane 7 : Human thymus lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
    • ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.

    Anti-Histone H2A.Z antibody [EPR6172] (ab124793) 使用論文

    ab124793 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 3000 cells
    Gel Running Conditions Reduced Denaturing (15%)
    Sample Human Cell lysate - nuclear (Melanoma)
    Specification Melanoma
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    投稿 Sep 01 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"