Anti-Histone H2A.Z 抗体 - ChIP Grade (ab97941)


  • 製品名Anti-Histone H2A.Z antibody - ChIP Grade
    Histone H2A.Z 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H2A.Z - ChIP Grade
  • アプリケーション適用あり: ICC/IF, ChIP, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide containing a sequence corresponding to a region within amino acids 1-15 (


    ) of Human Histone H2A.Z (NP_002097).

  • ポジティブ・コントロール
    • Jurkat whole cell lysate. ICC/IF: Hela cells




Our Abpromise guarantee covers the use of ab97941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
ChIP Use 2 µg for 25 µg of chromatin.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.


  • 機能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • 配列類似性Belongs to the histone H2A family.
  • 翻訳後修飾Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all

Anti-Histone H2A.Z antibody - ChIP Grade 画像

  • Anti-Histone H2A.Z antibody - ChIP Grade (ab97941) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size : 13 kDa
    15% SDS PAGE
  • Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab97941 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ab97941 staining Histone H2A.Z in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab97941 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H2A.Z staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.

  • Immunohistochemical staining of paraffin embedded SCC4 xenograft using ab97941 at 1/100.

Anti-Histone H2A.Z antibody - ChIP Grade (ab97941) 使用論文

ab97941 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 0.5 µg
Gel Running Conditions Reduced Denaturing (Criterion TGX 4-20% (BioRad))
Sample Human Recombinant protein (Recombinant octamers & purified K562 cell histones)
Specification Recombinant octamers & purified K562 cell histones
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Ragnhild Eskeland

Verified customer

投稿 Jan 27 2015