Anti-Histone H2A.Z 抗体 - ChIP Grade (ab4626)

製品の概要

  • 製品名
    Anti-Histone H2A.Z antibody - ChIP Grade
    Histone H2A.Z 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H2A.Z - ChIP Grade
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ChIP, WB, IPmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide corresponding to Saccharomyces cerevisiae Histone H2A.Z aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12692
    (Peptide available as ab17826)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
IP Use at an assay dependent dilution. PubMed: 17373856

ターゲット情報

  • 機能
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • 配列類似性
    Belongs to the histone H2A family.
  • 翻訳後修飾
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • 細胞内局在
    Nucleus. Chromosome.
  • Information by UniProt
  • 別名
    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all

画像

  • Anti-Histone H2A.Z antibody - ChIP Grade (ab4626) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 20 minutes


    Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ab4626 used in ChIP.
    Nuclear lysate prepared from S cerevisiae S288c was used.
    Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde.
    The detection step was performed using Real-time PCR.
    ab4626 was used at a 1/100 dilution for 4 hours at 4°C.

    See Abreview

参考文献

This product has been referenced in:
  • Ye B  et al. Suppression of SRCAP chromatin remodelling complex and restriction of lymphoid lineage commitment by Pcid2. Nat Commun 8:1518 (2017). Read more (PubMed: 29138493) »
  • Neves LT  et al. The histone variant H2A.Z promotes efficient cotranscriptional splicing inS. cerevisiae. Genes Dev 31:702-717 (2017). Read more (PubMed: 28446598) »

See all 17 Publications for this product

レビューと Q&A


I have aligned both protein sequences - the overall homology is 77%, but for the immunogen region (N-term.) there is unfortunately no homology. Thus, this antibody would not work with S.pombe.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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