The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Use at an assay dependent dilution. PubMed: 17373856
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Belongs to the histone H2A family.
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis. Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site. Not phosphorylated.
Predicted band size: 14 kDa Observed band size: 14 kDa
Exposure time: 20 minutes
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4626)Image courtesy of Harsh Ja by Abreview.
ab4626 used in ChIP. Nuclear lysate prepared from S cerevisiae S288c was used.Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde. The detection step was performed using Real-time PCR.ab4626 was used at a 1/100 dilution for 4 hours at 4°C.