Anti-Histone H2A.Z 抗体 [8H8] (ab80153)

製品の概要

  • 製品名Anti-Histone H2A.Z antibody [8H8]
    Histone H2A.Z 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [8H8] to Histone H2A.Z
  • アプリケーション適用あり: IHC-P, WB, Flow Cytmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human, Recombinant Fragment
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Histone H2A.Z.

  • ポジティブ・コントロール
    • This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名8H8
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 13.5 kDa.
Flow Cyt Use 1µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • 配列類似性Belongs to the histone H2A family.
    • 翻訳後修飾Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • 細胞内局在Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Anti-Histone H2A.Z antibody [8H8] 画像

    • All lanes : Anti-Histone H2A.Z antibody [8H8] (ab80153) at 10 µg/ml

      Lane 1 : Histone H2A.Z recombinant protein
      Lane 2 : Histone H2A recombinant protein

      Lysates/proteins at 0.5 µg per lane.

      Secondary
      Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Predicted band size : 13.5 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab80153 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP.  For further experimental details please refer to abreview.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab80153 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80153, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] ab18541, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Histone H2A.Z antibody [8H8] (ab80153) 使用論文

    ab80153 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Pancreatic cancer)
    Specification Pancreatic cancer
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Mar 11 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"