Anti-Histone H2A.X (phospho Y142) 抗体 (ab94602)


  • 製品名Anti-Histone H2A.X (phospho Y142) antibody
    Histone H2A.X 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H2A.X (phospho Y142)
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Cow, Human
    交差が予測される動物種: Dog, Macaque Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Histone H2A.X aa 100 to the C-terminus (phospho Y142) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H2A.X, phosphorylated at Y142.

    (Peptide available as ab113997)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Calf Thymus Histone (CTH) Preparation Nuclear Lysate.



Our Abpromise guarantee covers the use of ab94602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H2A.X (phospho Y142) peptide (ab113997).


  • 機能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
  • 配列類似性Belongs to the histone H2A family.
  • 発生段階Synthesized in G1 as well as in S-phase.
  • ドメインThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • 翻訳後修飾Phosphorylated on Ser-140 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).
    Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW228881 antibody
    • H2A histone family member X antibody
    • H2A.FX antibody
    • H2A.X antibody
    • H2a/x antibody
    • H2AFX antibody
    • H2AX antibody
    • H2AX histone antibody
    • H2AX_HUMAN antibody
    • Hist5.2ax antibody
    • Histone 2A antibody
    • Histone 2AX antibody
    • Histone H2A.X antibody
    • Histone H2AX antibody
    • RGD1566119 antibody
    see all

Anti-Histone H2A.X (phospho Y142) antibody 画像

  • All lanes : Anti-Histone H2A.X (phospho Y142) antibody (ab94602) at 1 µg/ml

    Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
    Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate with Histone H2A.X (phospho Y142) peptide (ab113997) at 1 µg/ml
    Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H2A.X peptide (ab114003) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 14 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab94602 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94602, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Histone H2A.X (phospho Y142) antibody (ab94602) 使用論文

ab94602 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Recombinant protein (Mouse Recombinant protein (C-terminus of H2A.X wit)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (Dot blot)
Loading amount 0.384 µg
Specification Mouse Recombinant protein (C-terminus of H2A.X wit
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Peter Ellis

Verified customer

投稿 Jun 02 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.15% · Temperature: RT°C
Sample Mouse Cell (Germ Cells)
Specification Germ Cells
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde

Abcam user community

Verified customer

投稿 Jul 24 2014

La référence du peptide utilisé pour produire ab94602 est Histone H2A.X (phospho Y142) peptide (ab113997).

Thank you very much for your interest our AbTrial program.

To our knowledge, ab94602 has not been tested in mouse. However by participating in our AbTrial program you can now use our products in an untested application or species without finan...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (OSTEOSARCOMA SAOS-2)
Fixative Paraformaldehyde
Permeabilization Yes - PBS-TRITON 0.25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Apr 18 2012