Anti-Histone H2A.X (phospho S139) 抗体 (Biotin) (ab113290)

製品の概要

  • 製品名Anti-Histone H2A.X (phospho S139) antibody (Biotin)
    Histone H2A.X 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H2A.X (phospho S139) (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Bat
  • 免疫原

    This information is considered to be commercially sensitive.

  • ポジティブ・コントロール
    • Whole cell lysate from etoposide treated HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 15 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
    • 配列類似性Belongs to the histone H2A family.
    • 発生段階Synthesized in G1 as well as in S-phase.
    • ドメインThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
    • 翻訳後修飾Phosphorylated on Ser-140 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).
      Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    • 細胞内局在Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AW228881 antibody
      • H2A histone family member X antibody
      • H2A.FX antibody
      • H2A.X antibody
      • H2a/x antibody
      • H2AFX antibody
      • H2AX antibody
      • H2AX histone antibody
      • H2AX_HUMAN antibody
      • Hist5.2ax antibody
      • Histone 2A antibody
      • Histone 2AX antibody
      • Histone H2A.X antibody
      • Histone H2AX antibody
      • RGD1566119 antibody
      see all

    Anti-Histone H2A.X (phospho S139) antibody (Biotin) 画像

    • All lanes : Anti-Histone H2A.X (phospho S139) antibody (Biotin) (ab113290) at 1 µg/ml

      Lane 1 : Whole cell lysate from etoposide treated HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from etoposide treated HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from etoposide treated HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 15 kDa


      Exposure time : 30 seconds

    Anti-Histone H2A.X (phospho S139) antibody (Biotin) (ab113290) 使用論文

    ab113290 has not yet been referenced specifically in any publications.

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